四、結論

油和(he)/或 Corexit 的(de)存在會導致(zhi) EPS 的(de)蛋白(bai)質：多糖(tang)(tang)比(bi)(bi)率(lv)(lv)更高(gao)，并(bing)在中胚層(ceng)實驗中降低 SFT。 在這些實驗中，SFT 與 蛋白(bai)質：具有負斜率(lv)(lv)的(de) EPS 多糖(tang)(tang)。 當開(kai)闊(kuo)的(de)海(hai)洋 水域(yu)和(he)兩種(zhong)不同的(de)沿海(hai)水處理進行了(le)比(bi)(bi)較， 蛋白(bai)質趨勢：多糖(tang)(tang)為 CEWAF > DCEWAF > WAF ≥ Control 并(bing)且對(dui)于 SFT，它是相反的(de)， CEWAF < DCEWAF < WAF ≤ 對(dui)照。 因此(ci)，SFT 與膠體 EPS 中的(de)蛋白(bai)質：多糖(tang)(tang)比(bi)(bi)率(lv)(lv)成反比(bi)(bi)。

當中宇宙水柱(zhu)的(de)不同(tong)尺寸分(fen)(fen)數(shu)為(wei) 相比(bi)之下，我(wo)們發現 EPS 膠體可以降(jiang)(jiang)低(di) SFT 蛋白(bai)質：多糖比(bi)例，表明有(you)效的(de)生物(wu)乳化 蛋白(bai)質的(de)容量。 粒子濾波中 SFT 的(de)比(bi)較 分(fen)(fen)數(shu) (< 0.45 μm) 和(he) EPS 膠體分(fen)(fen)數(shu) (< 0.45 μm 和(he) > 3 kDa)，對于真正(zheng)溶解(jie)的(de)部分(fen)(fen) (< 3 kDa)，它(ta)是 表明只有(you)前(qian)兩個(ge)包含(han) EPS 的(de)部分(fen)(fen)具有(you)容量 以降(jiang)(jiang)低(di) SFT，而 < 3 kDa 級分(fen)(fen)顯示與以下相同(tong)的(de) SFT 純(chun)海水或只有(you)真正(zheng)溶解(jie)有(you)機碳(tan)的(de)海水。

顯(xian)微(wei)鏡技(ji)術（即 CLSM 和 SEM）證實，正如預(yu)測(ce)的(de)(de)(de)(de)(de)(de)那(nei)樣，蛋白質(zhi)主要在空氣 - 水(shui)界面(mian)富集(ji)， 強烈影響空氣/水(shui)界面(mian)處的(de)(de)(de)(de)(de)(de) SFT 治療。 這(zhe)些(xie)技(ji)術還可視化(hua)(hua)了不同的(de)(de)(de)(de)(de)(de)聚集(ji)體尺寸 和它們的(de)(de)(de)(de)(de)(de)分(fen)散，以及聚集(ji)體形成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)重要性 通(tong)過(guo)陰離子(zi)EPS組(zu)分(fen)部分(fen)之間的(de)(de)(de)(de)(de)(de)Ca2+"橋接(jie)"。 SFT 可能會(hui)發生微(wei)小的(de)(de)(de)(de)(de)(de)變(bian)化(hua)(hua)，與(yu)(yu)蛋白質(zhi)：多(duo)糖比率的(de)(de)(de)(de)(de)(de)變(bian)化(hua)(hua)相(xiang)吻合(he)，這(zhe)可能是 pH 值變(bian)化(hua)(hua)的(de)(de)(de)(de)(de)(de)原因（十分(fen)之一） 單(dan)(dan)位），如 EPS 模(mo)型化(hua)(hua)合(he)物(wu)所示，這(zhe)可能在 CMC 周(zhou)圍最為突出。 此外(wai)，我們表明蛋白質(zhi)和酸性多(duo)糖的(de)(de)(de)(de)(de)(de) EPS 模(mo)型成(cheng)(cheng)分(fen)比 Corexit 導致海(hai)水(shui)中膠束的(de)(de)(de)(de)(de)(de)自組(zu)裝甚至 當這(zhe)些(xie)成(cheng)(cheng)分(fen)的(de)(de)(de)(de)(de)(de)濃(nong)度(du)很低時(shi)。 這(zhe)個 表明 EPS 在形成(cheng)(cheng)方面(mian)與(yu)(yu) Corexit 相(xiang)同或更有效 乳液。 然而，關于相(xiang)互作用的(de)(de)(de)(de)(de)(de)更系統的(de)(de)(de)(de)(de)(de)研究 不同組(zu)件(jian)的(de)(de)(de)(de)(de)(de)不同組(zu)合(he)，以及更多(duo)型號 單(dan)(dan)獨(du)的(de)(de)(de)(de)(de)(de)化(hua)(hua)合(he)物(wu)，可能需要更多(duo)地闡明在我們的(de)(de)(de)(de)(de)(de)中宇(yu)宙實驗中觀察到的(de)(de)(de)(de)(de)(de)復(fu)雜性。

致謝

這項研究得到(dao)了墨西哥灣(wan)(wan)的(de)(de)資助 支持名為 ADDOMEx 的(de)(de)聯盟(meng)研究的(de)(de)研究計劃 (微生物對分散劑和(he)油的(de)(de)聚集和(he)降解 Exopolymers) 聯盟(meng)。 原始數據可以在(zai)海灣(wan)(wan)找到(dao) 墨西哥研究倡議(yi)信息和(he)數據合作組(zu)織(zhi) (GRIIDC) 在(zai)網址 //doi.org/10.7266/N7PK0D64； //doi.org/10。 7266/N78P5XZD； //doi.org/10.7266/N74X568X； //doi. org/10.7266/N79W0D1K。

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蛋白質外聚物中多糖的比例——摘要、簡介

蛋白質外聚物中多糖的比例——方法

蛋白質外聚物中多糖的比例——結果與討論

蛋白質外聚物中多糖的比例——結論、致謝！