5.結論

首(shou)先，表面(mian)張力(li)測(ce)量證(zheng)明牛精漿具有(you)很(hen)好(hao)的(de)(de)表面(mian)活(huo)(huo)性，可(ke)能與(yu)(yu)BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)有(you)關。其次，Langmuir膜(mo)法(fa)(fa)（先前被證(zheng)明是公牛精子(zi)外(wai)(wai)細(xi)胞膜(mo)的(de)(de)相(xiang)關脂(zhi)質單(dan)層模型[35]）有(you)助于表征BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)對脂(zhi)質膜(mo)的(de)(de)親(qin)(qin)和力(li)。BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)能夠(gou)到達被磷(lin)(lin)脂(zhi)覆蓋的(de)(de)牛精子(zi)細(xi)胞表面(mian)，這得(de)益于它(ta)們(men)與(yu)(yu)磷(lin)(lin)脂(zhi)酰膽堿(jian)的(de)(de)相(xiang)互作(zuo)(zuo)用和自(zi)身的(de)(de)表面(mian)活(huo)(huo)性。我們(men)的(de)(de)假設是，由于它(ta)們(men)自(zi)身的(de)(de)表面(mian)活(huo)(huo)性，它(ta)們(men)首(shou)先穿透精子(zi)的(de)(de)外(wai)(wai)小葉，然后由于與(yu)(yu)磷(lin)(lin)脂(zhi)酰膽堿(jian)的(de)(de)強(qiang)烈相(xiang)互作(zuo)(zuo)用而留在(zai)那里。最后，利用Langmuir膜(mo)法(fa)(fa)對已知的(de)(de)BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)螯(ao)合劑如ldl和脂(zhi)質體的(de)(de)作(zuo)(zuo)用進行了篩(shai)選。脂(zhi)質體與(yu)(yu)低密度(du)脂(zhi)蛋(dan)白(bai)(bai)(bai)(bai)(bai)一(yi)樣有(you)效(xiao)地阻止BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)插(cha)入磷(lin)(lin)脂(zhi)層。低密度(du)脂(zhi)蛋(dan)白(bai)(bai)(bai)(bai)(bai)和脂(zhi)質體的(de)(de)比例相(xiang)同：0.16–0.17 mg磷(lin)(lin)脂(zhi)酰膽堿(jian)/mg BSP。我們(men)還不知道(dao)在(zai)冷(leng)凍保(bao)存(cun)的(de)(de)時間尺(chi)度(du)上脂(zhi)質體的(de)(de)非常微弱的(de)(de)插(cha)入是否有(you)一(yi)些生物學后果。需要進一(yi)步研究分析每種(zhong)BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)的(de)(de)表面(mian)性質及其在(zai)膜(mo)上的(de)(de)作(zuo)(zuo)用。此外(wai)(wai)，可(ke)以(yi)測(ce)試Langmuir膜(mo)，以(yi)檢測(ce)BSP與(yu)(yu)膜(mo)的(de)(de)親(qin)(qin)和力(li)隨(sui)溫度(du)的(de)(de)變(bian)化[20]。最后，Langmuir膜(mo)法(fa)(fa)是一(yi)種(zhong)有(you)效(xiao)的(de)(de)篩(shai)選BSP蛋(dan)白(bai)(bai)(bai)(bai)(bai)質新螯(ao)合劑的(de)(de)方(fang)法(fa)(fa)。該方(fang)法(fa)(fa)可(ke)適用于其他動物物種(zhong)。

致謝

作者(zhe)感(gan)謝IMV Technologies（法國）的財務(wu)支持，Gérard Chatagnon負(fu)責(ze)精漿蛋白質定量，Véronique Solé負(fu)責(ze)凝膠電泳，Maureen Collobert負(fu)責(ze)英語校正，Alain Sire和Patrice Papineau負(fu)責(ze)手套箱的構思和實現，允許(xu)在受控大(da)氣中使用朗繆爾槽。

附錄(lu)A.補充數(shu)據(ju)

 與本文(wen)相關的補充數據可在(zai)在(zai)線版本中找到，網(wang)址為(wei)//dx.doi.org/10.1016/j.colsurfb.2015.11.34

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